概述

  • 产品名称Anti-DOK5抗体
    参阅全部 DOK5 一抗
  • 描述
    兔多克隆抗体to DOK5
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: SKGPKRLEKF SDERAAYFRC YHKVTELNNV KNVARLPKST KKHAIGIYFN, corresponding to amino acids 36-85 of Human DOK5 (NP_060901).

  • 阳性对照
    • Placenta lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 功能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
  • 组织特异性Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
  • 序列相似性Belongs to the DOK family. Type B subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域PTB domain mediates receptor interaction.
  • 翻译后修饰Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.
  • Information by UniProt
  • 数据库链接
  • 别名
    • chromosome 20 open reading frame 180 antibody
    • Docking protein 5 antibody
    • DOK 5 antibody
    • DOK5 antibody
    • DOK5 protein antibody
    • DOK5_HUMAN antibody
    • Downstream of tyrosine kinase 5 antibody
    • Insulin receptor substrate 6 antibody
    • IRS-6 antibody
    • IRS6 antibody
    see all

Anti-DOK5 antibody 图像

  • Anti-DOK5 antibody (ab80367) at 1 µg/ml ( in 5% skim milk / PBS buffer) + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

Anti-DOK5 antibody (ab80367)参考文献

ab80367 has not yet been referenced specifically in any publications.

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