概述

  • 产品名称Anti-DOK3抗体
    参阅全部 DOK3 一抗
  • 描述
    小鼠多克隆抗体to DOK3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human DOK3 protein (AAH04564.2; Q7L591-3).

  • 阳性对照
    • Human liver tissue lysate, DOK3 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab89327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa for isoform 1 and 36 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
    • 组织特异性Expressed in spleen.
    • 序列相似性Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 结构域PTB domain mediates receptor interaction.
    • 翻译后修饰Constitutively tyrosine-phosphorylated.
      On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
    • 细胞定位Cytoplasm. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Docking protein 3 antibody
      • DOK 3 antibody
      • DOK Like Protein antibody
      • Dok3 antibody
      • DOK3_HUMAN antibody
      • DOKL Pending antibody
      • Downstream of tyrosine kinase 3 antibody
      • p62 DOK Like Protein antibody
      see all

    Anti-DOK3 antibody 图像

    • Anti-DOK3 antibody (ab89327) at 1 µg/ml + human liver tissue lysate at 50 µg

      Predicted band size : 36 kDa
      Observed band size : ~58 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DOK3 antibody (ab89327) at 1 µg/ml

      Lane 1 : DOK3 transfected 293T cell lysate
      Lane 2 : Non transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    Anti-DOK3 antibody (ab89327)参考文献

    ab89327 has not yet been referenced specifically in any publications.

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