概述

  • 产品名称
  • 描述
    山羊多克隆抗体to DOK3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    TRERRKGPAPCDRP

    , corresponding to C terminal amino acids 483-490 of Human DOK3.

  • 阳性对照
    • Human PBMC lysate.
  • 常规说明
    GenBank Accession Number – NP_079148.

性能

应用

Our Abpromise guarantee covers the use of ab1357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 57 kDa).
    Peptide ELISA: antibody detection limit dilution >1/32000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
    • 组织特异性
      Expressed in spleen.
    • 序列相似性
      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 结构域
      PTB domain mediates receptor interaction.
    • 翻译后修饰
      Constitutively tyrosine-phosphorylated.
      On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
    • 细胞定位
      Cytoplasm. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Docking protein 3 antibody
      • DOK 3 antibody
      • DOK Like Protein antibody
      • Dok3 antibody
      • DOK3_HUMAN antibody
      • DOKL Pending antibody
      • Downstream of tyrosine kinase 3 antibody
      • p62 DOK Like Protein antibody
      see all

    图片

    • Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg

      Predicted band size : 57 kDa


      Primary incubated for 1 hour. Detected by chemiluminescence.

    实验方案

    文献

    This product has been referenced in:
    • Bounab Y  et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . Read more (PubMed: 23820730) »
    • Heckel T  et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . Read more (PubMed: 19164586) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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