Anti-DOK1 (phospho Y398)抗体(ab75741)

概述

  • 产品名称Anti-DOK1 (phospho Y398)抗体
    参阅全部 DOK1 一抗
  • 描述
    兔多克隆抗体to DOK1 (phospho Y398)
  • 特异性ab75741 detects endogenous levels of DOK1 only when phosphorylated at tyrosine 398.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).

  • 阳性对照
    • Human breast carcinoma tissue. Extracts from K562 cells, starved.

性能

应用

Our Abpromise guarantee covers the use of ab75741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域The PTB domain mediates receptor interaction.
  • 翻译后修饰Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho Y398) antibody 图像

  • ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution

    Lane 1 : K562 cell extracts, unstarved
    Lane 2 : K562 cell extracts, starved


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-DOK1 (phospho Y398) antibody (ab75741)参考文献

ab75741 has not yet been referenced specifically in any publications.

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