Anti-DOK1 (phospho Y362)抗体(ab76173)

概述

  • 产品名称Anti-DOK1 (phospho Y362)抗体
    参阅全部 DOK1 一抗
  • 描述
    兔多克隆抗体to DOK1 (phospho Y362)
  • 特异性The sequence used as immunogen has high homology to DOK2 (Tyr-337). The site is not conserved in other DOK family members.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to amino acids surrounding tyrosine 362 in human DOK1, coupled to KLH.

  • 阳性对照
    • Jurkat cells stimulated with calyculin A (100 nM, 30 min).

性能

应用

Our Abpromise guarantee covers the use of ab76173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
ELISA 1/2000.

靶标

  • 功能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域The PTB domain mediates receptor interaction.
  • 翻译后修饰Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho Y362) antibody 图像

  • All lanes : Anti-DOK1 (phospho Y362) antibody (ab76173) at 1/1000 dilution

    Lane 1 : Jurkat cells stimulated with calyculin A (100 nM, 30 min)
    Lane 2 : Jurkat cells stimulated with calyculin A (100 nM, 30 min), treated with alkaline phosphatase


    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

Anti-DOK1 (phospho Y362) antibody (ab76173)参考文献

ab76173 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76173.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"