Anti-DOK1 (phospho Y362)抗体(ab75742)

概述

  • 产品名称Anti-DOK1 (phospho Y362)抗体
    参阅全部 DOK1 一抗
  • 描述
    兔多克隆抗体to DOK1 (phospho Y362)
  • 特异性ab75742 endogenous levels of DOK1 only when phosphorylated at tyrosine 362.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A Synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 362 (P-I-YP-D-E).

  • 阳性对照
    • Human breast carcinoma tissue and extracts from Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab75742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域The PTB domain mediates receptor interaction.
  • 翻译后修饰Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho Y362) antibody 图像

  • ab75742, at a 1/50 dilution, staining DOK1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing phospho peptide.
  • All lanes : Anti-DOK1 (phospho Y362) antibody (ab75742) at 1/500 dilution

    Lane 1 : Jurkat cell extracts with immunising peptide
    Lane 2 : Jurkat cell extracts


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

Anti-DOK1 (phospho Y362) antibody (ab75742)参考文献

ab75742 has not yet been referenced specifically in any publications.

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