Anti-DOK1 (phospho S450)抗体(ab76172)

概述

  • 产品名称Anti-DOK1 (phospho S450)抗体
    参阅全部 DOK1 一抗
  • 描述
    兔多克隆抗体to DOK1 (phospho S450)
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phospho-peptide corresponding to amino acids surrounding serine 450 from human DOK1, conjugated to KLH.

  • 阳性对照
    • Jurkat cells stimulated with calyculin
  • 常规说明Do not aliquot ab76172

性能

应用

Our Abpromise guarantee covers the use of ab76172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
ELISA 1/2000.

靶标

  • 功能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域The PTB domain mediates receptor interaction.
  • 翻译后修饰Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho S450) antibody 图像

  • All lanes : Anti-DOK1 (phospho S450) antibody (ab76172) at 1/1000 dilution

    Lane 1 : Jurkat cells stimulated with calyculin A (100 nM, 30 min)
    Lane 2 : Jurkat cells stimulated with calyculin A (100 nM, 30 min) followed by lambda phosphatase.


    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

Anti-DOK1 (phospho S450) antibody (ab76172)参考文献

ab76172 has not yet been referenced specifically in any publications.

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