概述

  • 产品名称
  • 描述
    兔多克隆抗体to DOK1
  • 经测试应用
    适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 425-439 of Human DOK1 (Peptide available as ab39756.)

  • 阳性对照
    • Jurkat whole cell lysate
  • 常规说明
    Tyrosine kinase substrate p62DOK


    Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.

性能

应用

Our Abpromise guarantee covers the use of ab8112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with Human DOK1 peptide (ab39756).
ICC/IF Use a concentration of 2 µg/ml.
IP Use at an assay dependent dilution. (see Abreview published on 27th July, 2006).

靶标

  • 功能
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性
    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域
    The PTB domain mediates receptor interaction.
  • 翻译后修饰
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位
    Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 antibody 图像

  • All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/ml

    Lane 1 : Jurkat cell lysates
    Lane 2 : THP-1 cell lysate


    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF

Anti-DOK1 antibody (ab8112)参考文献

This product has been referenced in:
  • Siouda M  et al. Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog 10:e1004125 (2014). WB . Read more (PubMed: 24809689) »
  • Barrett A  et al. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. J Cell Sci 127:2647-58 (2014). Read more (PubMed: 24762811) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Jurkat cells)
Total protein in input
2e+007 cells
Specification
Jurkat cells
Immuno-precipitation step
Protein A
Username

Mr. Guittard Geoffrey

Verified customer

提交于 Jul 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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