概述

  • 产品名称
  • 描述
    兔多克隆抗体to DOK1
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    KTWAVLYPASPHGVAR

    conjugated to KLH, corresponding to amino acids 24-39 of Human DOK1.

  • 阳性对照
    • HL-60 cell lysate, Human breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab37835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

靶标

  • 功能
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 组织特异性
    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • 序列相似性
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 结构域
    The PTB domain mediates receptor interaction.
  • 翻译后修饰
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • 细胞定位
    Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

图片

  • Anti-DOK1 antibody (ab37835) at 1/100 dilution + HL-60 cell lysate.

    Secondary
    HRP-anti-rabbit

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab37835, at 1/1000 dilution, staining DOK1 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

实验方案

文献

ab37835 has not yet been referenced specifically in any publications.

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