RabMAb

Anti-DOCK2抗体[EPR6647] (ab124838)

概述

  • 产品名称Anti-DOCK2抗体[EPR6647]
    参阅全部 DOCK2 一抗
  • 描述
    兔单克隆抗体[EPR6647] to DOCK2
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human DOCK2 protein (Q92608).

  • 阳性对照
    • Jurkat and Raji cell lysates and Human lung tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 212 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is not recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 相关性DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.
    • 细胞定位Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
    • 数据库链接
    • 别名
      • Dedicator of cyto kinesis 2 antibody
      • Dedicator of cytokinesis 2 antibody
      • Dedicator of cytokinesis protein 2 antibody
      • DOCK 2 antibody
      • FLJ46592 antibody
      • KIAA0209 antibody
      see all

    Anti-DOCK2 antibody [EPR6647] 图像

    • All lanes : Anti-DOCK2 antibody [EPR6647] (ab124838) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 212 kDa
    • ab124838, at 1/50 - 1/100, staining DOCK2 in Human lung tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    Anti-DOCK2 antibody [EPR6647] (ab124838)参考文献

    ab124838 has not yet been referenced specifically in any publications.

    Product Wall

    You are correct, we observed better staining in IHC-P with ab124838 when no antigen retrieval was used. Thus, we recommend skipping the antigen retrieval step in your protocol. This may vary depending on your tissue type and processing methods, so it m...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"