概述

  • 产品名称Anti-DOCK2抗体
    参阅全部 DOCK2 一抗
  • 描述
    兔多克隆抗体to DOCK2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1800 to the C-terminus of Human DOCK2.

    (Peptide available as ab74657.)

  • 阳性对照
    • This antibody gave a positive signal in Jurkat Whole Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab64449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 212 kDa (predicted molecular weight: 212 kDa).

靶标

  • 相关性DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.
  • 细胞定位Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
  • 数据库链接
  • 别名
    • Dedicator of cyto kinesis 2 antibody
    • Dedicator of cytokinesis 2 antibody
    • Dedicator of cytokinesis protein 2 antibody
    • DOCK 2 antibody
    • FLJ46592 antibody
    • KIAA0209 antibody
    see all

Anti-DOCK2 antibody 图像

  • Anti-DOCK2 antibody (ab64449) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 212 kDa
    Observed band size : 212 kDa


    Exposure time : 5 minutes
  • IHC image of ab64449 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64449, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DOCK2 antibody (ab64449)参考文献

ab64449 has not yet been referenced specifically in any publications.

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