The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
组织特异性Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
序列相似性Belongs to the DOCK family. Contains 1 DHR-1 (CZH-1) domain. Contains 1 DHR-2 (CZH-2) domain. Contains 1 SH3 domain.
结构域The DHR-2 domain is necessary and sufficient for the GEF activity.
细胞定位Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
Detection of DOCK1 by Western Blot of Immunprecipitate.
anti-DOCK1 at 1µg/ml staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab75278 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
Anti-DOCK1 antibody (ab75278)参考文献
has not yet been referenced specifically in any publications.