概述

性能

应用

Our Abpromise guarantee covers the use of ab1348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    Western Blot – Approx 150-200kDa band seen in A431 cell lysate.
    Recommended for use at 3-4µg/ml.
  • 靶标

    • 功能
      Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
    • 组织特异性
      Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
    • 序列相似性
      Belongs to the DOCK family.
      Contains 1 DHR-1 (CZH-1) domain.
      Contains 1 DHR-2 (CZH-2) domain.
      Contains 1 SH3 domain.
    • 结构域
      The DHR-2 domain is necessary and sufficient for the GEF activity.
    • 细胞定位
      Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 180 kDa protein downstream of CRK antibody
      • Ced5 antibody
      • Dedicator of Cytokinesis 1 antibody
      • Dedicator of cytokinesis protein 1 antibody
      • DOCK 1 antibody
      • DOCK 180 antibody
      • DOCK1 antibody
      • DOCK1_HUMAN antibody
      • DOCK180 antibody
      see all

    Anti-DOCK1 antibody 图像

    • DOCK1 staining (4µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence

    实验方案

    Anti-DOCK1 antibody (ab1348)参考文献

    ab1348 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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