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Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
Our Abpromise guarantee covers the use of ab3493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1000 - 1/3000. 1/1000 - 1/3000 against 0.1µg of the immunizing peptide.|
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 43.6 kDa. Use at an assay dependent dilution. The target may need to be immunoprecipitated to identify the target in WB. Predicted molecular weight: 43.6 kDa.|
Transfection of U2OS cells (10cm dish) using 3
Note: ab3493 worked in WB following IP, but did not work in a straight WB.
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