概述

  • 产品名称Anti-Dnmt3L抗体
    参阅全部 Dnmt3L 一抗
  • 描述
    兔多克隆抗体to Dnmt3L
  • 特异性This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse.
  • 经测试应用适用于: ICC/IF, ELISA, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
    Sequence:

    C-GLLQRRRKWRSQL


    Database link: Q9UJW3

  • 常规说明

    DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
  • Primary antibody说明DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
ELISA 1/1000 - 1/3000. 1/1000 - 1/3000 against 0.1µg of the immunizing peptide.
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 43.6 kDa. Use at an assay dependent dilution. The target may need to be immunoprecipitated to identify the target in WB. Predicted molecular weight: 43.6 kDa.
IP 1/100.

靶标

  • 功能Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
  • 组织特异性Expressed at low levels in several tissues including testis, ovary, and thymus.
  • 序列相似性Belongs to the C5-methyltransferase family.
    Contains 1 ADD-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosine 5 methyltransferase 3 like protein antibody
    • DNA (cytosine 5 ) methyltransferase 3 like antibody
    • DNA (cytosine-5)-methyltransferase 3-like antibody
    • DNA cytosine 5 methyltransferase 3 like protein antibody
    • DNA methyltransferase 3 like protein antibody
    • DNM3L_HUMAN antibody
    • Dnmt 3L antibody
    • Dnmt3l antibody
    • Human cytosine 5 methyltransferase 3 like protein antibody
    • MGC1090 antibody
    see all

Anti-Dnmt3L antibody 图像

  • Transfection of U2OS cells (10cm dish) using 3µg of GAL4-DNMT3L. Protein extraction using IPH 150mM. IP and WB conditions, see Fuks et al. (2000). Nature Genetics 24: 88-91.WB using 0.4µg/ml in TBS milk 2%, BSA 0.5%. Dectection using ECL.

    Note: ab3493 worked in WB following IP, but did not work in a straight WB.

  • Anti-Dnmt3L antibody (ab3493) at 1/1000 dilution + Rat C6 cells, whole cell lysate at 25 µg

    Secondary
    HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43.6 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunofluorescence analysis of 2-cell mouse embryos, staining Dnmt3L with ab3493.

    Embryos were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 0.5% blocking reagent in TNT (0.1 M, Tris-HCl, pH 7.5, 0.15 M NaCl, 0.05% Tween-20) buffer for 20 min at 4°C. Embryos were incaubted with primary antibody (1/500) for 1 hour at 37°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/400) was used as the secondary antibody.

Anti-Dnmt3L antibody (ab3493)参考文献

This product has been referenced in:
  • Lee SM  et al. HBx induces hypomethylation of distal intragenic CpG islands required for active expression of developmental regulators. Proc Natl Acad Sci U S A 111:9555-60 (2014). Mouse . Read more (PubMed: 24941955) »
  • Yang J  et al. Stem Cell Gene SALL4 Suppresses Transcription through Recruitment of DNA Methyltransferases. J Biol Chem 287:1996-2005 (2012). WB ; Human . Read more (PubMed: 22128185) »

See all 5 Publications for this product

Product Wall

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I can confirm that it was not experimentally tested if this antibody shows any cross-reaction with the proteins DNMT3A and/or DNMT3B.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (lymphoma cell line)
Loading amount 150 µg
Specification lymphoma cell line
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (rat lung tissue)
Loading amount 30 µg
Specification rat lung tissue
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 14 2009

Application Western blot
Sample Armenian Hamster Tissue lysate - whole (hamster muscle tissue)
Loading amount 20 µg
Specification hamster muscle tissue
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (lung tissue)
Total protein in input 100 µg
Specification lung tissue
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 Mar 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (lung tissue)
Total protein in input 100 µg
Specification lung tissue
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 Mar 09 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"