Anti-Dnmt3L抗体(ab28968)

概述

  • 产品名称
  • 描述
    鸡多克隆抗体to Dnmt3L
  • 特异性
    ab28968 recognises Dnmt3L.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Dnmt3L.
    Database link: Q9UJW3

  • 阳性对照
    • Purified recombinant Human Dnmt3L.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯化说明
    ab28968 is a crude IgY preparation from egg yolks using a precipitation method (PEG, Polyethylene Glycol).
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能
    Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
  • 组织特异性
    Expressed at low levels in several tissues including testis, ovary, and thymus.
  • 序列相似性
    Belongs to the C5-methyltransferase family.
    Contains 1 ADD-type zinc finger.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosine 5 methyltransferase 3 like protein antibody
    • DNA (cytosine 5 ) methyltransferase 3 like antibody
    • DNA (cytosine-5)-methyltransferase 3-like antibody
    • DNA cytosine 5 methyltransferase 3 like protein antibody
    • DNA methyltransferase 3 like protein antibody
    • DNM3L_HUMAN antibody
    • Dnmt 3L antibody
    • Dnmt3l antibody
    • Human cytosine 5 methyltransferase 3 like protein antibody
    • MGC1090 antibody
    see all

Anti-Dnmt3L antibody 图像

  • Anti-Dnmt3L antibody (ab28968) at 1/500 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

实验方案

Anti-Dnmt3L antibody (ab28968)参考文献

ab28968 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Hela cells)
Loading amount
40 µg
Specification
Hela cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Sep 16 2009

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