概述

  • 产品名称
  • 描述
    兔多克隆抗体to Dnmt3b
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Chinese hamster
    预测可用于: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Dnmt3b.

    (Peptide available as ab16376.)

  • 阳性对照
    • This antibody gave a positive result in the following whole cell lysates: CHO, HUES7, F9, Mouse Embryonic Germ Cell, E14Tg2a.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.8 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). Abcam recommends using milk as the blocking agent (3-5%). Please contact Scientific Support for more information.

靶标

  • 功能
    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
  • 组织特异性
    Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
  • 疾病相关
    Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
  • 序列相似性
    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • 结构域
    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻译后修饰
    Sumoylated.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosine 5methyltransferase 3B antibody
    • DNA antibody
    • DNA (cytosine 5) methyltransferase 3 beta antibody
    • DNA (cytosine 5)-methyltransferase 3B antibody
    • DNA (cytosine-5)-methyltransferase 3B antibody
    • DNA methyltransferase HsaIIIB antibody
    • DNA MTase HsaIIIB antibody
    • DNM3B_HUMAN antibody
    • Dnmt3b antibody
    • EC 2.1.1.37 antibody
    • ICF antibody
    • ICF1 antibody
    • M.HsaIIIB antibody
    • MGC124407 antibody
    • RP23-89H14.3 antibody
    see all

Anti-Dnmt3b antibody 图像

  • All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/ml (blocked with 3% milk.)

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : Mouse Embryonic Germ Cell Lysate
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa


    Exposure time : 2 minutes
  • All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/ml

    Lane 1 : CHO cell lysate
    Lane 2 : CHO cell lysate with Mouse Dnmt3b peptide (ab16376) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit Alexa fluor 680 at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
  • All lanes : Anti-Dnmt3b antibody (ab16049) at 1/1000 dilution

    Lane 1 : Lysate prepared from human NCCIT cell chromatin fraction
    Lane 2 : Lysate prepared from chromatin fraction of human NCCIT whole cells

    Lysates/proteins at 200000 cells per lane.

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 seconds

    This image is a courtesy of Johanna Smith

    See Abreview

Anti-Dnmt3b antibody (ab16049)参考文献

This product has been referenced in:
  • Sharma S  et al. Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone. J Biol Chem 291:19351-63 (2016). Read more (PubMed: 27422817) »
  • Fang X  et al. cAMP induces hypertrophy and alters DNA methylation in HL-1 cardiomyocytes. Am J Physiol Cell Physiol 309:C425-36 (2015). Mouse . Read more (PubMed: 26224577) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Jul 03 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Jun 27 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Jun 27 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (GI, SMC)
Gel Running Conditions
Reduced Denaturing (4% upper gel and 10% lower gel run at 100V for 100 minutes)
Loading amount
50 µg
Specification
GI, SMC
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Dr. Seungil Ro

Verified customer

提交于 Apr 05 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 14 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Rat Tissue (Dorsal Root Ganglia)
Specification
Dorsal Root Ganglia
Username

Sarah Pollema

Verified customer

提交于 Oct 16 2012

DISCOUNT CODE: ****
Expiration date: January 21, 2013

I am very pleased to hear you would like to accept our offer and test ab16049 on rat tissue. This code will give you one free primary antibody before the expiration date. To redeem t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary)
Specification
Ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 25 2010

Application
Western blot
Sample
Human Cell lysate - other (NCCIT cells - mediastinal mixed germ cell tumor)
Loading amount
200000 cells
Specification
NCCIT cells - mediastinal mixed germ cell tumor
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Johanna Smith

Verified customer

提交于 Aug 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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