概述

  • 产品名称Anti-Dnmt3a抗体
    参阅全部 Dnmt3a 一抗
  • 描述
    兔多克隆抗体to Dnmt3a
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken
  • 免疫原

    Synthetic peptide corresponding to Human Dnmt3a aa 457-486 conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • WB: T247-D cell lysate. IHC-P: Human hepatocarcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/400.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 101 kDa.

靶标

  • 功能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • 组织特异性Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • 序列相似性Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • 结构域The PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻译后修饰Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • 细胞定位Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3 alpha antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • OTTHUMP00000201149 antibody
    • TBRS antibody
    see all

Anti-Dnmt3a antibody 图像

  • Anti-Dnmt3a antibody (ab4897) + T47-D cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling Dnmt3a with ab4897.

Anti-Dnmt3a antibody (ab4897)参考文献

This product has been referenced in:
  • Elabd C  et al. DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells. J Cell Biol 203:73-85 (2013). Read more (PubMed: 24127215) »
  • Chik F & Szyf M Effects of specific DNMT gene depletion on cancer cell transformation and breast cancer cell invasion; toward selective DNMT inhibitors. Carcinogenesis 32:224-32 (2011). WB ; Human . Read more (PubMed: 20980350) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Testis
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 14 2015

Thank you for your phone call.
I am sorry that the antibody is not working as expected. As we discussed, I have arranged for a free of charge replacement with ab4897. Please see details below. The item is currently backordered, but the estimated de...

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Thank you very much for your interest in ab4897.

To our knowledge, ab4897 has not been tested in Mouse and Rat.It is very likely that ab4897works in these species since thehomolgy between the immunogen peptide and the proteins of interest is 1...

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Thank you for your reply.
I am sorry to confirm it is not possible to provide a blocking peptide on this occasion. I apologise for the inconvenience, I understand this would have been useful in this case.

I hope the other suggestions pr...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

I would like to reassure you that these antibodies are tested and covered by our 6 month guarantee fo...

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (lung carcinoma)
Total protein in input 300 µg
Specification lung carcinoma
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hESC, mESC)
Specification hESC, mESC
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS for 15min at room temp.
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2007

The originator used T47-D cell lysate as a positive control for ab4897 in Western blotting. If you have any more questions, please let us know.

To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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The antibody has not been tested for its reactivity with either of these therefore we have no further information on this.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"