概述

  • 产品名称
  • 描述
    兔多克隆抗体to Dnmt3a
  • 经测试应用
    适用于: WB, IP, EMSAmore details
    不适用于: Neut
  • 种属反应性
    与反应: Chicken, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 10-118 of Human Dnmt3a.

  • 阳性对照
    • HCT116 whole cell lysate.
  • 常规说明
    It is strongly suggested that Sodium Azide is added to this stock upon thawing and storage at 4 degrees C.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
EMSA
  • 应用说明
    EMSA: 2ul antiserum is used in PMID 19046370.
    IP: 1/1000.
    WB: 1/1000. Detects a band of approximately 85 kDa.

    Is unsuitable for Neut.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
    • 组织特异性
      Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
    • 序列相似性
      Belongs to the C5-methyltransferase family.
      Contains 1 ADD domain.
      Contains 1 GATA-type zinc finger.
      Contains 1 PHD-type zinc finger.
      Contains 1 PWWP domain.
    • 结构域
      The PWWP domain is essential for targeting to pericentric heterochromatin.
    • 翻译后修饰
      Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
    • 细胞定位
      Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA (cytosine 5) methyltransferase 3 alpha antibody
      • DNA (cytosine 5) methyltransferase 3A antibody
      • DNA (cytosine-5)-methyltransferase 3A antibody
      • DNA cytosine methyltransferase 3A2 antibody
      • DNA methyltransferase 3 alpha antibody
      • DNA methyltransferase 3a antibody
      • DNA methyltransferase HsaIIIA antibody
      • DNA MTase HsaIIIA antibody
      • DNM3A_HUMAN antibody
      • DNMT 3a antibody
      • DNMT antibody
      • Dnmt3a antibody
      • DNMT3A2 antibody
      • M.HsaIIIA antibody
      • MCMT antibody
      • OTTHUMP00000201149 antibody
      • TBRS antibody
      see all

    Anti-Dnmt3a antibody (ab16704)参考文献

    This product has been referenced in:
    • Yossifoff M  et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci 28:2267-77 (2008). WB, EMSA ; Chicken . Read more (PubMed: 19046370) »
    • Loriot A  et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem 281:10118-26 (2006). Read more (PubMed: 16497664) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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