Anti-Dnmt3a抗体(ab16704)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Dnmt3a抗体
参阅全部 Dnmt3a 一抗 -
描述
兔多克隆抗体to Dnmt3a -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to amino acids 10-118 of Human Dnmt3a.
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阳性对照
- ICC: Ntera-2 whole cell lysate. IHC-P: Human testis tissue
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常规说明
It is strongly suggested that Sodium Azide is added to this stock upon thawing and storage at 4 degrees C.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16704于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/200.
|
|
ICC |
1/500.
|
说明 |
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IHC-P
1/200. |
ICC
1/500. |
靶标
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功能
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
组织特异性
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
序列相似性
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
结构域
The PWWP domain is essential for targeting to pericentric heterochromatin. -
翻译后修饰
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
细胞定位
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 1788 Human
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- Unigene: 515840 Human
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别名
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
图片
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Immunocytochemistry analysis of Ntera-2 cells labeling Dnmt3a with ab16704 followed by Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Immunohistochemistry analysis of human testis labeling Dnmt3a with ab16704 at 1/200. Hematoxylin was used as counterstain.
数据表及文件
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Datasheet download
文献 (7)
ab16704 被引用在 7 文献中.
- Szigeti KA et al. Global DNA hypomethylation of colorectal tumours detected in tissue and liquid biopsies may be related to decreased methyl-donor content. BMC Cancer 22:605 (2022). PubMed: 35655145
- Huang YH et al. Systematic Profiling of DNMT3A Variants Reveals Protein Instability Mediated by the DCAF8 E3 Ubiquitin Ligase Adaptor. Cancer Discov N/A:N/A (2021). PubMed: 34429321
- Abdalla BA et al. A Novel Dnmt3a1 Transcript Inhibits Adipogenesis. Front Physiol 9:1270 (2018). PubMed: 30333755
- Yossifoff M et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci 28:2267-77 (2008). WB, EMSA ; Chicken . PubMed: 19046370
- Loriot A et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem 281:10118-26 (2006). PubMed: 16497664
- Robert MF et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet 33:61-5 (2003). PubMed: 12496760
- Beaulieu N et al. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J Biol Chem 277:28176-81 (2002). PubMed: 12015329