概述

  • 产品名称Anti-Dnmt3a抗体
    参阅全部 Dnmt3a 一抗
  • 描述
    山羊多克隆抗体to Dnmt3a
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Dog, Physarum polycephalum
  • 免疫原

    Synthetic peptide:

    DKNATKAGDDEPEY

    by a Cysteine residue linker, corresponding to internal sequence amino acids 271-284 of Human Dnmt3a (NP_072046.2; NP_715640.2; NP_783329.1).

  • 阳性对照
    • Human and Rat liver lysates.
  • 常规说明


    ab113430 is expected to recognize reported isoforms a (NP_072046.2) and isoform b (NP_715640.2). Reported variants represent identical protein: NP_072046.2, NP_783328.1. ab113430 is not expected to recognize Dnmt3B.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab113430 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明ab113430 is expected to recognize reported isoforms a (NP_072046.2) and isoform b (NP_715640.2). Reported variants represent identical protein: NP_072046.2, NP_783328.1. ab113430 is not expected to recognize Dnmt3B.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa). Primary incubation time: 1 hour.

靶标

  • 功能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • 组织特异性Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • 序列相似性Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • 结构域The PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻译后修饰Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • 细胞定位Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3 alpha antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • OTTHUMP00000201149 antibody
    • TBRS antibody
    see all

Anti-Dnmt3a antibody 图像

  • Anti-Dnmt3a antibody (ab113430) at 0.5 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 102 kDa

Anti-Dnmt3a antibody (ab113430)参考文献

ab113430 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"