概述

  • 产品名称Anti-Dnmt3a抗体[64B814]
    参阅全部 Dnmt3a 一抗
  • 描述
    小鼠单克隆抗体[64B814] to Dnmt3a
  • 经测试应用适用于: WB, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Bacteria expressed HIS-tag recombinant mouse Dnmt3a.

  • 阳性对照
    • 293 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号64B814
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

靶标

  • 功能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • 组织特异性Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • 序列相似性Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • 结构域The PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻译后修饰Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • 细胞定位Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3 alpha antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • OTTHUMP00000201149 antibody
    • TBRS antibody
    see all

Anti-Dnmt3a antibody [64B814] 图像



  • Predicted band size : 102 kDa


    Western blot analysis for Dnmt3a using ab13887 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).

Anti-Dnmt3a antibody [64B814] (ab13887)参考文献

This product has been referenced in:
  • Sato N  et al. The orphan nuclear receptor GCNF recruits DNA methyltransferase for Oct-3/4 silencing. Biochem Biophys Res Commun 344:845-51 (2006). WB ; Mouse . Read more (PubMed: 16631596) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"