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Bacteria expressed HIS-tag recombinant mouse Dnmt3a.
Abcam is committed to meeting high standards of manufacturing including guaranteeing lot-to-lot consistency and security of supply. We have decided to discontinue this product within the next few months as we are unable to secure its future high-quality supply. We are sorry for any inconvenience this may cause. We would recommend antibody ab188470 as a replacement.
Our Abpromise guarantee covers the use of ab13888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Use at a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Dnmt3a Transfected and Untransfected Cell Lysate (ab90154) is the recommended positive control for WB.|
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration. PubMed: 27956497|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immuno-staining of Dnmt3a using ab13888 at 5 ug/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
Western blot analysis for Dnmt3a using ab13888 at 2 ug/ml against 10
Formalin-fixed, paraffin-embedded human liver tissue stained for Dnmt3a using ab13888 at 5 µg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Dnmt3a using ab13888 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of (A) CHO (chinese hamster ovary cell line) and (B) HEK-293 (human epithelial cell line from embryonic kidney) cell lines labeling Dnmt3a with ab13888 at 1 µg/106 cells (dark blue) compared with an isotype control (orange).
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