概述

  • 产品名称DNMT1 Inhibitor Screening Assay试剂盒
    参阅全部 DNMT1 试剂盒
  • 检测类型Enzyme activity
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase. The selective inhibition of DNMT1 may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT1 inhibitors could be a new addition to cancer therapeutic agents.


    Abcam's DNMT1 Inhibitor Screening Assay Kit (ab113465) allows the user to selectively screen DNMT1 inhibitors quickly. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 activity/inhibitor screening experiment without the need for radioactivity or any special equipment.


    Not sure if this is the right product for you? Check out our DNMT Product Guide for help.

  • 说明

    It might be necessary to purchase DNMT1 enzyme - we recommend Recombinant human DNMT1 protein (ab198109).

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab113465 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    DNMT1 Inhibitor Screening Assay Kit 图像

    • Recombinant DNMT1 was incubated with substrate and inhibitors. DNMT1 activity was measured using ab113465 in the presence or absence of inhibitors.

    实验方案

    DNMT1 Inhibitor Screening Assay Kit (ab113465)参考文献

    This product has been referenced in:
    • Tong Z  et al. Age-related formaldehyde interferes with DNA methyltransferase function, causing memory loss in Alzheimer's disease. Neurobiol Aging 36:100-10 (2015). Read more (PubMed: 25282336) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"