概述

  • 产品名称Anti-Dnmt1抗体[EPR3522]
    参阅全部 Dnmt1 一抗
  • 描述
    兔单克隆抗体[EPR3522] to Dnmt1
  • 经测试应用适用于: WB, IP, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues at the C-terminus of Human Dnmt1.

  • 阳性对照
    • HuT-78, Jurkat or 293T lysate HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Dnmt1 antibody (Phycoerythrin) [EPR3522] (ab208999)

性能

  • 形式Liquid
  • 存放��明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR3522
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab92314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 183 kDa.
IP 1/20.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
    • 组织特异性Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
    • 序列相似性Belongs to the C5-methyltransferase family.
      Contains 2 BAH domains.
      Contains 1 CXXC-type zinc finger.
    • 结构域The N-terminal part is required for homodimerization and acts as a regulatory domain.
    • 翻译后修饰Sumoylated; sumoylation increases activity.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADCADN antibody
      • AIM antibody
      • CXXC finger protein 9 antibody
      • CXXC-type zinc finger protein 9 antibody
      • CXXC9 antibody
      • DNA (cytosine 5 ) methyltransferase 1 antibody
      • DNA (cytosine-5)-methyltransferase 1 antibody
      • DNA methyltransferase 1 antibody
      • DNA methyltransferase HsaI antibody
      • DNA methyltransferase M.HsaI. antibody
      • DNA MTase antibody
      • DNA MTase HsaI antibody
      • DNMT 1 antibody
      • DNMT antibody
      • Dnmt1 antibody
      • DNMT1_HUMAN antibody
      • Dnmt1o antibody
      • FLJ16293 antibody
      • HSN1E antibody
      • M.HsaI antibody
      • MCMT antibody
      • Met1 antibody
      • MGC104992 antibody
      • mMmul antibody
      • MommeD2 antibody
      see all

    Anti-Dnmt1 antibody [EPR3522] 图像



    • Predicted band size : 183 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: DNMT1 knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HEK293 whole cell lysate (20 µg)
      Lane 4: HeLa whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92314 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab92314 was shown to specifically react with DNMT1 when DNMT1 knockout samples were used. Wild-type and DNMT1 knockout samples were subjected to SDS-PAGE.  Ab92314 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • Overlay histogram showing HeLa cells stained with ab92314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92314, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Dnmt1 antibody [EPR3522] (ab92314) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 183 kDa
    • Immunofluorescence staining of Jurkat cells with purified ab92314 at a working dilution of 1/2000, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    Anti-Dnmt1 antibody [EPR3522] (ab92314)参考文献

    This product has been referenced in:
    • Clements EG  et al. DNMT1 modulates gene expression without its catalytic activity partially through its interactions with histone-modifying enzymes. Nucleic Acids Res 40:4334-46 (2012). WB . Read more (PubMed: 22278882) »

    See 1 Publication for this product

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