Anti-Dnmt1抗体- ChIP Grade (ab87656)


  • 产品名称Anti-Dnmt1抗体- ChIP Grade
    参阅全部 Dnmt1 一抗
  • 描述
    兔多克隆抗体to Dnmt1 - ChIP Grade
  • 经测试应用适用于: WB, IP, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1037-1386 of Mouse Dnmt1, purified by SDS-PAGE.

  • 阳性对照
    • Murine erythrolekemia cells MEL11A2



Our Abpromise guarantee covers the use of ab87656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 183 kDa.
IP Use at an assay dependent dilution. Especially good for denatured protein.
ICC/IF 1/2000 - 1/5000. Background can be lowered by treatment of sample with SDS/TritonX-100 mixture (1:5 weight ratio).
ChIP Use at an assay dependent dilution.


  • 功能Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • 组织特异性Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • 序列相似性Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • 结构域The N-terminal part is required for homodimerization and acts as a regulatory domain.
  • 翻译后修饰Sumoylated; sumoylation increases activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all

Anti-Dnmt1 antibody - ChIP Grade 图像

  • All lanes : Anti-Dnmt1 antibody - ChIP Grade (ab87656) at 1 µg/ml

    Lane 1 : lysate from Mouse erythrolekemia cells MEL11A2
    Lane 2 : lysate from untransfected Mouse myoblast cells C2C12
    Lane 3 : lysate from C2C12 cells transfected with Dnmt1 overexpressing plasmid
    Lane 4 : lysate from C2C12 cells transfected with negative control plasmid

    Predicted band size : 183 kDa
  • Lysate from Mouse erythrolekemia cells MEL11A2 (lane 1), untransfected Mouse myoblast cells C2C12 (lane 2) , C2C12 cells transfected with Dnmt1 overexpressing plasmid (lane 3) and C2C12 cells transfected with negative control plasmid (lane 4) were immunoprecipitated under denaturing conditions using ab87656.
    The immunoprecipitae was subsequently loaded on a gel and probed with ab87656 at 1yg/ml.

Anti-Dnmt1 antibody - ChIP Grade (ab87656)参考文献

This product has been referenced in:
  • Meadows JP  et al. Dynamic DNA methylation regulates neuronal intrinsic membrane excitability. Sci Signal 9:ra83 (2016). Read more (PubMed: 27555660) »
  • Wang KY  et al. Epigenetic Enhancement of the Post-replicative DNA Mismatch Repair of Mammalian Genomes by a Hemi-(m)CpG-Np95-Dnmt1 Axis. Sci Rep 6:37490 (2016). IP ; Mouse . Read more (PubMed: 27886214) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (mouse embryonic stem cells)
Permeabilization Yes - 0.1% Tween (PBS)
Gating Strategy P1+single-cells
Specification mouse embryonic stem cells
Fixation IC fixation buffer

Liliana Antunes

Verified customer

提交于 Nov 30 2016

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We will guarantee our antibodies for any species and applications listed as tested on our datasheets, even if the reviews are negative. If for any reason you are unsatisfied with your results when using one of our...

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The anti-Dnmt1 antibody ab87656 was used for ChIP with mouse embryonic stem cells. I hope this is helpful. Please contact us again if you have any further questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Mammary gland, liver, mammary gland tumor)
Loading amount 50 µg
Specification Mammary gland, liver, mammary gland tumor
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

提交于 Jun 17 2011