概述

  • 产品名称
  • 描述
    兔多克隆抗体to Dnmt1
  • 特异性
    ab87656 is recommended for DNMT1 detection in WB.
  • 经测试应用
    适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form

  • 阳性对照
    • WB: HeLa cell nuclear extracts.
  • 常规说明


    Useful for analysis of the protein complex containing Dnmt1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, traces of Ammonium sulphate, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Useful for analysis of the protein complex containing Dnmt1.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution. 1 ug of the antibody can precipitates 0.5~1.0 ug of Dnmt1. Antibody works best for native Dnmt1.
ICC/IF 1/5000.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa).

靶标

  • 功能
    Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • 组织特异性
    Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • 序列相似性
    Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • 结构域
    The N-terminal part is required for homodimerization and acts as a regulatory domain.
  • 翻译后修饰
    Sumoylated; sumoylation increases activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all

图片

  • ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
  • All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml

    Lane 1 : Tagged-NP 95 + BirA Input
    Lane 2 : Tagged-NP 95 + BirA Beads
    Lane 3 : BirA Input
    Lane 4 : BirA Beads


    Predicted band size : 183 kDa
    Observed band size : 183 kDa
  • All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml

    Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
    Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
    Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
    Lane 4 : BirA (0.1M KCl fraction)


    Predicted band size : 183 kDa

文献

This product has been referenced in:
  • Zhou FC  et al. Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum. PLoS One 11:e0162063 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27583369) »
  • Thakur A  et al. Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin 9:53 (2016). WB ; Mouse . Read more (PubMed: 27895716) »

See all 6 Publications for this product

客户评价及客户问答

The cell extract, containinjg about 10 ug protein, is applied to each lane in SDS-PAGE and 0.2˜1.0 ug/ml of the antibody are used in WB. There is no special protocol needed for using this antibody. However, it should be kept in mind that Dnmt1 protein ...

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Thank you for contacting us.

I am sorry to hear that the antibody is not working as expected.

As we discussed over the phone, please let me or my colleague xxx know if the 1/5000 dilution and using mouse samples improved your resul...

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Thank you for contacting us. I am waiting for confirmation from the laboratory to confirm that the Dnmt1 antibody has been tested on Hela by ICC and will send this to you when I receive it, assuming it exists. If there is no data, I do still think it w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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