概述

  • 产品名称
  • 描述
    兔多克隆抗体to Dnmt1
  • 特异性
    Dnmt 1. Will recognize the splice isoforms B and C.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to within the last 100 amino acids of Human Dnmt 1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified through a protein G column and eluted out with both high and low pH buffers, neutralized immediately after elution, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 183 kDa.

靶标

  • 功能
    Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • 组织特异性
    Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • 序列相似性
    Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • 结构域
    The N-terminal part is required for homodimerization and acts as a regulatory domain.
  • 翻译后修饰
    Sumoylated; sumoylation increases activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all

图片

  • ab4870 used in Western blot to detect Dnmt1 in Jurkat cell lysate.

实验方案

文献

This product has been referenced in:
  • Sarkar S  et al. Histone Deacetylase Inhibitors Reverse CpG Methylation by Regulating DNMT1 through ERK Signaling. Anticancer Res 31:2723-32 (2011). WB ; Human . Read more (PubMed: 21868513) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your reply.

I am not sure what the lower bands might represent. They are a bit low to represent any of the isoforms of DNMT1 in rat. A BLAST of the immunogen sequence did not reveal any homology with another protein that may be d...

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Thank you for contacting Abcam.

I am sorry this product did not perform in rat as you had hoped and for the inconvenience this has caused. Since this antibody has not been tested in rat, we do not know if and how well it might work in this sam...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cultured Hepatoma Cell Line)
Specification
Cultured Hepatoma Cell Line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton-X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 27 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Rat Cell lysate - whole cell (Liver)
Specification
Liver
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
ab5208 DNMT1 ChIP
Negative control
Rabbit IgG
Username

Abcam user community

Verified customer

提交于 Nov 27 2006

Thank you for your patience and I'm sorry to hear that you are experiencing difficulty with these antibodies. All the information that we have regarding these antibodies is located on the online datasheets; as these three antibodies were originated out...

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The peptide sequence used as the immunogen is CSASAKIKEEEAAKD corresponding to the C-term of human Dnmt1 (NP_001370).

The immunogen is a synthetic peptide conjugated to KLH, corresponding to within the last 100 amino acids of Human Dnmt 1. From analysing this sequence using Swiss Prot and BLAST I can see there is a 97% similarity between the human sequence and bovine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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