Anti-DNMBP抗体(ab88534)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to DNMBP
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    不与反应: Xenopus laevis
  • 免疫原

    Full length human DNMBP protein (AAH41628.1).

  • 阳性对照
    • Hela cell lysate; DNMBP transfected 293T cell lysate Hela cell

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa for isoform 1, and 93 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
    • 组织特异性
      Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
    • 序列相似性
      Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.
    • 细胞定位
      Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    图片

    • Anti-DNMBP antibody (ab88534) at 1 µg/ml + Hela cell lysate at 50 µg

      Predicted band size : 177 , 93 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DNMBP antibody (ab88534) at 1 µg/ml

      Lane 1 : DNMBP transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 177 , 93 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    • ab88534, at 10µg/ml, staining DNMBP in Hela cell by Immunofluorescence.

    文献

    ab88534 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    500 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Stage 11 and Stage 32 Embryos)
    Specification
    Stage 11 and Stage 32 Embryos
    Blocking step
    KPL Blocking Reagent as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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