antigen sequence: KHSQATQPML ILLPPPGHPS ATLHPLTVIQ KLAAKYQQGQ KQLQVIALGS EAWDPVSVVV STLSQAMYEG HWLVLDNCHL MPHW, corresponding to internal sequence amino acids 4080-4163 of Human DNHD1.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the dynein heavy chain family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- C11orf47 antibody
- CCDC35 antibody
- coiled-coil domain containing 35 antibody
Immunocytochemistry/ Immunofluorescence - Anti-DNHD1 antibody (ab122574)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab122574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNHD1 antibody (ab122574)
ab122574, at 1/50 dilution, staining DNHD1 in paraffin-embedded Human lung tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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