This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

概述

性能

应用

  • 应用说明
    This antibody gave a positive result in ELISA against the immunizing peptide (ab39926).
    Not yet tested in other applications.

    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells (By similarity). Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs. Sequence-specific RNA-binding factor that binds specifically to U-rich regions (URRs) in the 3' untranslated region (3'-UTR) of several mRNAs. Does not bind to miRNAs. May play a role during primordial germ cell (PGC) survival (By similarity). However, does not seem to be essential for PGC migration.
    • 序列相似性
      Contains 2 RRM (RNA recognition motif) domains.
    • 细胞定位
      Nucleus. Cytoplasm. Perinuclear germ granules, also called germ plasm or chromatoid body. Colocalizes in perinuclear sites with APOBEC3.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dead end homolog 1 (zebrafish) antibody
      • dead end homolog 1 antibody
      • Dead end protein antibody
      • Dead end protein homolog 1 antibody
      • Dead end, zebrafish, homolog, 1 antibody
      • DND microRNA-mediated repression inhibitor 1 antibody
      • dnd1 antibody
      • DND1_HUMAN antibody
      • MGC34750 antibody
      • RBMS4 antibody
      • RNA binding motif single stranded interacting protein 4 antibody
      • RNA-binding motif antibody
      • single-stranded-interacting protein 4 antibody
      • Ter antibody
      see all

    Anti-Dnd1 antibody 图像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • IHC image of Dnd1 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39928, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Anti-Dnd1 antibody (ab39928)参考文献

    ab39928 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa Jurkat HEK293)
    Loading amount
    20 µg
    Specification
    HeLa Jurkat HEK293
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Dec 24 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (14.5 dpc fetal testes)
    Specification
    14.5 dpc fetal testes
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.1M citrate pH6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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