概述

  • 产品名称Anti-DNASE2B抗体
    参阅全部 DNASE2B 一抗
  • 描述
    兔多克隆抗体to DNASE2B
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: WMAQRLKTHL LTETWQRKRQ ELPSNCSLPY HVYNIKAIKL SRHSYFSSYQ, corresponding to amino acids 254-303 of Human DNASE2B

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab66738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 19, 34 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

靶标

  • 相关性DNASE2B shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this protein is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene.
  • 细胞定位Lysosome
  • 数据库链接
  • 别名
    • Deoxyribonuclease 2 beta antibody
    • Deoxyribonuclease 2 beta precursor antibody
    • Deoxyribonuclease II beta antibody
    • DLAD antibody
    • DNase 2 like acid DNase antibody
    • DNASE 2B antibody
    • DNase II beta antibody
    • DNase II like acid DNase antibody
    • DNASE2 antibody
    • DNase2 like acid DNase antibody
    • DnaseIIb antibody
    • EC 3.1.22.1 antibody
    • Endonuclease DLAD antibody
    • Lysosomal DNase II antibody
    • UOX antibody
    see all

Anti-DNASE2B antibody 图像

  • Anti-DNASE2B antibody (ab66738) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa,~70 kDa. We are unsure as to the identity of these extra bands.

Anti-DNASE2B antibody (ab66738)参考文献

ab66738 has not yet been referenced specifically in any publications.

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