Anti-DNase gamma抗体(ab152118)
Key features and details
- Rabbit polyclonal to DNase gamma
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DNase gamma抗体
参阅全部 DNase gamma 一抗 -
描述
兔多克隆抗体to DNase gamma -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 115-305 of Human DNase gamma (UniProt; Q13609).
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阳性对照
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab152118于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units. -
组织特异性
Liver and spleen. -
序列相似性
Belongs to the DNase I family. -
细胞定位
Nucleus. May first pass through the ER membrane before being imported in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1776 Human
- Omim: 602244 Human
- SwissProt: Q13609 Human
- Unigene: 476453 Human
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别名
- Deoxyribonuclease gamma antibody
- Deoxyribonuclease I like 3 antibody
- Deoxyribonuclease I like III antibody
see all
图片
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DNase IL3 antibody [N3C3] detects DNase IL3 protein at cytoplasm and nucleus by immunofluorescent analysis. HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNase IL3 stained by DNase IL3 antibody diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. -
Anti-DNase gamma antibody (ab152118) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 36 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling DNase gamma with ab152118 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab152118 被引用在 2 文献中.
- Chen M et al. Fragmentomics of urinary cell-free DNA in nuclease knockout mouse models. PLoS Genet 18:e1010262 (2022). PubMed: 35793278
- Lazzaretto B & Fadeel B Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells. J Immunol 203:2276-2290 (2019). PubMed: 31519860