Anti-DNA/RNA Damage抗体[15A3] (ab62623)

概述

  • 产品名称Anti-DNA/RNA Damage抗体[15A3]
    参阅全部 DNA/RNA Damage 一抗
  • 描述
    小鼠单克隆抗体[15A3] to DNA/RNA Damage
  • 特异性15A3 binds with high specificity and affinity to oxo8dG (8-hydroxy-2'-deoxyguanosine), oxo8Gua (8-oxo-7,8-dihydroguanine), and oxo8G (8-oxo-7,8-dihydroguanosine) present in biological fluids with Kaff values of 9.2 x 108 M-1, 4.7 x' 17 M-1, and 2.1 x 108 M-1, respectively.
  • 经测试应用适用于: IHC-P, ELISA, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to DNA/RNA Damage. 8-hydroxy-guanosine-BSA and -casein conjugates.

  • 常规说明

    Please see the protocol booklet link below for recommended IHC and ICC staining procedure

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号15A3
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 10 µg/ml.

Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum.

ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

PubMed 25479606

IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21824519

靶标

  • 相关性In intact animals, lesions (adducts) excised from DNA are transported from the cell through the circulation and excreted in urine. In bacteria, DNA adducts are excreted directly into the medium. In either case, the adducts can be assayed as a measure of oxidative damage to DNA. In particular, Oxo-8-dG (8-Oxo-7,8-dihydro-2'-deoxyguanosine) serves as an excellent marker for DNA damage produced by oxidants because it represents one of the major products generated by a wide array of treatments associated with oxidant damage such as that produced by irradiation and various carcinogens and because it is implicated in spontaneous transversion mutagenesis. Oxo-8-Gua (8-oxo-7,8-dihydroguanine) is one of the most common DNA lesions resulting from reactive oxygen species and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome. In humans, it is primarily repaired by DNA glycosylase OGG1. It can be caused by ionizing radiation, in connection with oxidative metabolism. Oxo-8-G (8-oxo-7,8-dihydroguanosine) is classified as an oxidized ribonucleotide, and is primarily used in studies of oxidative RNA damage and associated RNA repair and RNA turnover mechanisms within the cell. In the cell, Oxo-8-G RNA lesions are formed by reaction with reactive oxygen species (ROS) generated either via normal oxidative metabolic processes, UV ionizing radiation, or exposure to oxidative agents. Oxidative RNA damage can lead to defects in protein synthesis, for example, decreased rates of protein synthesis and production of aggregated or truncated peptides, with important implications in aging and neurodegenerative disorders and artherosclerosis.
  • 别名
    • 7,8-Dihydro-8-oxo-2'-deoxyguanosine antibody
    • 7,8-Dihydro-8-oxodeoxyguanosine antibody
    • 7,8-Dihydro-8-oxoguanine antibody
    • 8-Hydroxy-2'-deoxyguanosine antibody
    • 8-Hydroxydeoxyguanosine antibody
    • 8-hydroxyguanine antibody
    • 8-OH-dG antibody
    • 8-Oxo-2'-deoxyguanosine antibody
    • 8-Oxo-7,8-dihydro-2'-deoxyguanosine (Oxo-8-dG) antibody
    • 8-Oxo-7,8-dihydrodeoxyguanosine antibody
    • 8-oxo-7,8-dihydroguanine (Oxo-8-Gua) antibody
    • 8-oxo-7,8-dihydroguanosine (Oxo-8-G) antibody
    • 8-Oxo-dG antibody
    • 8-Oxo-G antibody
    • 8-oxo-Gua antibody
    • 8-Oxo-guanosine antibody
    • 8-Oxo-riboguanosine antibody
    • 8-Oxoguanine antibody
    • OH8Gua antibody
    see all

Anti-DNA/RNA Damage antibody [15A3] 图像

  • Immunohistochemical analysis of murine brain tissue 24 hours after recirculation following ischemia. Staining using ab62623 at 1/1000 dilution. An AlexaFluor®488-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.

  • Left panel: ab62623 staining in ischemic rat brain tissue
    Centre panel: DAPI staining
    Right panel: merged

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.

    See Abreview

  • ab62623 staining in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.

    See Abreview

实验方案

Anti-DNA/RNA Damage antibody [15A3] (ab62623)参考文献

This product has been referenced in:
  • Lood C  et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nat Med N/A:N/A (2016). IF . Read more (PubMed: 26779811) »
  • Chung HK  et al. The indole derivative NecroX-7 improves nonalcoholic steatohepatitis in ob/ob mice through suppression of mitochondrial ROS/RNS and inflammation. Liver Int 35:1341-53 (2015). Read more (PubMed: 25443620) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization Yes - 0.1% Triton X-100 in TBS for 5-10 minutes
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 09 2016

Application Immunocytochemistry
Sample Oncorhynchus mykiss Cell (Spermatozoa)
Permeabilization Yes - 0,3%Triton-X100 in PBS1X
Specification Spermatozoa
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 4°C
Fixative Paraformaldehyde
Username

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Verified customer

提交于 Oct 06 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Specification Bone tissue (Femur)
Blocking step Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Mr. Helder Fonseca

Verified customer

提交于 Sep 11 2015

Application ELISA
Sample Human Cell (immortalized proximal tubule HK2 cells)
Specification immortalized proximal tubule HK2 cells
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 24°C
Type Other
Username

Abcam user community

Verified customer

提交于 Jul 22 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Mouse Cell (Hepatocyte)
Specification Hepatocyte
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 23 2014

We added IHC of paraffin-embedded tissue to the applications section of the ab62623 datasheet based on reviews we received from customers. These are linked on the main online datasheet. Each customer used a different method for antigen retrieval. One u...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (B16F10 melanoma)
Specification B16F10 melanoma
Fixative 4% Formalin
Permeabilization Yes - 0.1% SDS
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Mar 15 2013

Thank you for contacting us.

Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

Regarding ab23722, human colon ...

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Thank you for your reply.

Could you please confirm the order number so I can raise the new free order for ab62623?

We appreciate any feedback from customers using our products. The suggested tips aimed to help you solve the problems...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2012

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