概述

  • 产品名称Anti-DNALI1抗体
    参阅全部 DNALI1 一抗
  • 描述
    兔多克隆抗体to DNALI1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (VTANKAHTGQ GSCWVATLAS AMIPPADSLL KYDTPVLVSR NTEKRSPKAR) of Human DNALI1 (NP_003453).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20��C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May play a dynamic role in flagellar motility.
  • 组织特异性Expressed in many tissues. A smaller 0.9 kb and a larger 2.5 kb transcripts were detected at the highest level in the testis, at medium levels in the prostate, heart, liver, lung and pancreas, at low levels in the ovary, skeletal muscle and small intestine. Not detected in spleen, colon epithelium, thymus or peripheral blood leukocytes. The 0.9 kb transcript is expressed at a 20-fold higher level than the 2.5 kb transcript in the testis.
  • 序列相似性Belongs to the inner dynein arm light chain family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • axonemal antibody
    • Axonemal dynein light chain (hp28) antibody
    • Axonemal dynein light chain antibody
    • Axonemal dynein light intermediate polypeptide 1 antibody
    • dJ423B22.5 antibody
    • DNALI 1 antibody
    • DNALI1 antibody
    • Dynein axonemal light intermediate chain 1 antibody
    • Dynein axonemal light intermediate polypeptide 1 antibody
    • hp28 antibody
    • IDLC_HUMAN antibody
    • Inner dynein arm homolog of clamydomonas antibody
    • Inner dynein arm light chain antibody
    • Inner dynein arm light chain axonemal antibody
    • P28 antibody
    see all

Anti-DNALI1 antibody 图像

  • Species+tissue/cell type:Human nasal epithelial cells. Primary antibody dilution: 1:1000. Secondary antibody: Goat anti-rabbit Alexa Fluor 546. Secondary antibody dilution:1:1000

  • Anti-DNALI1 antibody (ab87075) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-DNALI1 antibody (ab87075)参考文献

This product has been referenced in:
  • Miao C  et al. Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans. G3 (Bethesda) 6:3307-3316 (2016). ICC/IF . Read more (PubMed: 27543293) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"