概述

  • 产品名称Anti-DnaK抗体[7D9D7]
    参阅全部 DnaK 一抗
  • 描述
    小鼠单克隆抗体[7D9D7] to DnaK
  • 经测试应用适用于: IHC-P, Electron Microscopy, ELISA, WBmore details
  • 种属反应性
    Reacts with mycobacterial HSP70 and cross-reactive with E. coli DnaK.
  • 免疫原

    DnaK from Mycobacterium avium ssp. paratuberculosis (MAP).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号7D9D7
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.

靶标

  • 相关性DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates.
  • 细胞定位Cytoplasm. Cell inner membrane; Peripheral membrane protein.
  • 别名
    • Chaperone Hsp70 antibody
    • Chaperone protein dnaK antibody
    • Co chaperone with DnaJ antibody
    • dnaK antibody
    • Heat shock 70 kDa protein antibody
    • HSP70 antibody
    see all

Anti-DnaK antibody [7D9D7] (ab73473)参考文献

This product has been referenced in:
  • Santema W  et al. Hsp70 vaccination-induced antibodies recognize B cell epitopes in the cell wall of Mycobacterium avium subspecies paratuberculosis. Vaccine : (2010). Read more (PubMed: 21199702) »
  • Burt SA  et al. Carvacrol induces heat shock protein 60 and inhibits synthesis of flagellin in Escherichia coli O157:H7. Appl Environ Microbiol 73:4484-90 (2007). Read more (PubMed: 17526792) »

See all 3 Publications for this product

Product Wall

Thank you and your customer for providing the sequence of DnaK from Xanthomonas campestris pv. Campestris. Both antibodies were generated with the full-length DnaK protein; ab69617 with E.coli DnaK and ab73473 with DnaK from Mycobacterium avium ssp....

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Thank you for your inquiry. Unfortunately, I do not have access to the sequence of DnaK of Xanthomonas campestris pv. Campestris. Could you please ask your customer to provide me with this sequence? I will then make an alignment. If the homolo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"