概述
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产品名称Anti-DNAJC9抗体
参阅全部 DNAJC9 一抗 -
描述兔多克隆抗体to DNAJC9
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宿主Rabbit
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经测试应用适用于: WB, IPmore details
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种属反应性与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
A region between residues 210 and 260 of human DNAJC9 (NP_056005.12)
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阳性对照
- HeLa whole cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab85958 was affinity purified using an epitope specific to DNAJC9 immobilized on solid support.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab85958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
靶标
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序列相似性Contains 1 J domain.
- Information by UniProt
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数据库链接
- Entrez Gene: 23234 Human
- Omim: 611206 Human
- SwissProt: Q8WXX5 Human
- Unigene: 408577 Human
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别名
- DnaJ homolog subfamily C member 9 antibody
- DnaJ Hsp40 homolog subfamily C member 9 antibody
- DnaJ protein SB73 antibody
see all
图片
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Western blot - DNAJC9 antibody (ab85958)All lanes : Anti-DNAJC9 antibody (ab85958) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 37 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 seconds -
Immunoprecipitation - DNAJC9 antibody (ab85958)Detection of DNAJC9 by Western Blot of Immunprecipitate. ab85958 at 1µg/ml staining DNAJC9 in HeLa whole cell lysate immunoprecipitated using ab85958 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. Observed band size : 37 kDa
文献
ab85958 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"