概述

  • 产品名称Anti-DNAJC9抗体
    参阅全部 DNAJC9 一抗
  • 描述
    兔多克隆抗体to DNAJC9
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    A region between residues 210 and 260 of human DNAJC9 (NP_056005.12)

  • 阳性对照
    • HeLa whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab85958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

Anti-DNAJC9 antibody 图像

  • All lanes : Anti-DNAJC9 antibody (ab85958) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 30 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of DNAJC9 by Western Blot of Immunprecipitate. ab85958 at 1µg/ml staining DNAJC9 in HeLa whole cell lysate immunoprecipitated using ab85958 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. Observed band size : 37 kDa

Anti-DNAJC9 antibody (ab85958)参考文献

ab85958 has not yet been referenced specifically in any publications.

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