The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.