概述

  • 产品名称Anti-DNAJC21抗体
    参阅全部 DNAJC21 一抗
  • 描述
    兔多克隆抗体to DNAJC21
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

应用

Our Abpromise guarantee covers the use of ab86434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May act as a co-chaperone for HSP70.
    • 组织特异性Brain, placenta, kidney and pancreas.
    • 序列相似性Contains 2 C2H2-type zinc fingers.
      Contains 1 J domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DJC21_HUMAN antibody
      • DnaJ homolog subfamily A member 5 antibody
      • DnaJ homolog subfamily C member 21 antibody
      • DNAJA5 antibody
      • dnajc21 antibody
      • GS3 antibody
      • JJJ1 antibody
      • Protein GS3 antibody
      see all

    Anti-DNAJC21 antibody 图像

    • All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 62 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.

    Anti-DNAJC21 antibody (ab86434)参考文献

    ab86434 has not yet been referenced specifically in any publications.

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