antigen, corresponding to amino acids 633-764 of Human DNAJC16 (UniProt Q9Y2G8).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 1 J domain.
Contains 1 thioredoxin domain.
Information by UniProt
DnaJ homolog subfamily C member 16 antibody
Immunocytochemistry/ Immunofluorescence - Anti-DNAJC16 antibody (ab122855)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122855 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-DNAJC16 antibody (ab122855)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC16 antibody (ab122855)
ab122855, at a 1/50 dilution, staining DNAJC16 in paraffin embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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