Anti-DNAJB9抗体(ab6053)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to DNAJB9
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    RGNMVTTYTDCSGQ

    , corresponding to C terminal amino acids 210-223 of Human MDG1.

  • 阳性对照
    • Human kidney
  • 常规说明
    GenBank Accession Number – NP_036460.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/4000. This antibody gave a positive result in ELISA against the immunizing peptide.
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa. Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1µg/ml.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • ab6053 at 4 µg/ml staining MDG1 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Detected with HRP-staining.

文献

ab6053 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us for technical support. I'm sorry to hear that ab6053 is not working well for you. This antibody is a FastTrack product and as such has not been fully characterised yet by our laboratory as we do not have good positive con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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