重组Anti-DNAJB6抗体[EPR17122] - N-terminal (ab198995)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17122] to DNAJB6 - N-terminal
- Suitable for: Flow Cyt (Intra), IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-DNAJB6抗体[EPR17122] - N-terminal
参阅全部 DNAJB6 一抗 -
描述
兔单克隆抗体[EPR17122] to DNAJB6 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IP, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- U87-MG, SH-SY5Y, HeLa and Jurkat lysate. Mouse heart, rat brain, rat heart, C6, RAW264.7 and PC-12 lysate. Human testis tissue. Rat cerebral cortex tissue. SH-SY5Y and Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17122 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198995于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/150.
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/250.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/150. |
WB
1/1000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/250. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity. -
组织特异性
Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus. -
疾病相关
Limb-girdle muscular dystrophy 1E -
序列相似性
Contains 1 J domain. -
结构域
The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus. -
细胞定位
Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. - Information by UniProt
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数据库链接
- Entrez Gene: 10049 Human
- Entrez Gene: 23950 Mouse
- Entrez Gene: 362293 Rat
- Omim: 611332 Human
- SwissProt: O75190 Human
- SwissProt: O54946 Mouse
- SwissProt: Q6AYU3 Rat
- Unigene: 188591 Human
see all -
别名
- DJ4 antibody
- DnaJ antibody
- DnaJ (Hsp40) homolog subfamily B member 6 antibody
see all
图片
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All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa -
Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/10000 dilution + Jurkat cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa -
All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat heart lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa -
Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.
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Intracellular Flow Cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730)IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
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DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg.
Lane 2: ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (8)
ab198995 被引用在 8 文献中.
- Weihl CC et al. Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure. Acta Neuropathol 145:127-143 (2023). PubMed: 36264506
- Qian FY et al. A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy. Acta Neuropathol Commun 9:23 (2021). PubMed: 33557929
- Han C et al. Porcine DNAJB6 promotes PCV2 replication via enhancing the formation of autophagy in host cells. Vet Res 51:61 (2020). PubMed: 32381067
- Rodríguez-González C et al. Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation. Sci Rep 10:8130 (2020). PubMed: 32424160
- Bengoechea R et al. Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy. J Clin Invest 130:4470-4485 (2020). PubMed: 32427588
- Cao YQ et al. Hsp40 Protein DNAJB6 Interacts with Viral NS3 and Inhibits the Replication of the Japanese Encephalitis Virus. Int J Mol Sci 20:N/A (2019). PubMed: 31739611
- Aprile FA et al. The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress a-synuclein aggregation. Sci Rep 7:9039 (2017). PubMed: 28831037
- Ding Y et al. A modifier screen identifies DNAJB6 as a cardiomyopathy susceptibility gene. JCI Insight 1: (2016). PubMed: 27642634