概述

  • 产品名称Anti-DNAJB6抗体
    参阅全部 DNAJB6 一抗
  • 描述
    兔多克隆抗体to DNAJB6
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Drosophila melanogaster
  • 免疫原

    A synthetic peptide corresponding to a region of Human DNAJB6

  • 阳性对照
    • Fetal muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab75196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36, 50 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

靶标

  • 功能Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
  • 组织特异性Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
  • 疾病相关Limb-girdle muscular dystrophy 1E
  • 序列相似性Contains 1 J domain.
  • 结构域The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
  • 细胞定位Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DJ4 antibody
    • DnaJ antibody
    • DnaJ (Hsp40) homolog subfamily B member 6 antibody
    • DNAJ B6 antibody
    • DnaJ homolog subfamily B member 6 antibody
    • DnaJ like 2 protein antibody
    • DNAJB6 antibody
    • DNJB6_HUMAN antibody
    • Heat shock protein J2 antibody
    • HHDJ1 antibody
    • HSJ-2 antibody
    • HSJ2 antibody
    • LGMD1E antibody
    • MRJ antibody
    • MSJ-1 antibody
    • MSJ1 antibody
    see all

Anti-DNAJB6 antibody 图像



  • Predicted band size : 36 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling DNAJB6 with ab75196 at 1.0µg/ml.
  • Anti-DNAJB6 antibody (ab75196) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Predicted band size : 36 kDa
    Observed band size : 36,50 kDa (why is the actual band size different from the predicted?)

Anti-DNAJB6 antibody (ab75196)参考文献

ab75196 has not yet been referenced specifically in any publications.

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