概述

  • 产品名称Anti-DNAJB2抗体
    参阅全部 DNAJB2 一抗
  • 描述
    小鼠单克隆抗体to DNAJB2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment: LELSRREQQP SVTSRSGGTQ VQQTPASCPL DSDLSEDEDL QLAMAYSLSE MEAAGKKPAG GREAQHRRQG RPKAQHQDPG LGGTQEGARG EATKRSPSPE EKASRCLIL, corresponding to amino acids 216-324 of Human DNAJB2 (NP_006727) with a tag.

  • 阳性对照
    • A431 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 0.3ng/ml as a capture antibody when used against the immunogen.

靶标

  • 相关性DNAJB2 is involved in heat shock protein and unfolded protein binding.
  • 数据库链接
  • 别名
    • Descriptions antibody
    • DnaJ (Hsp40) homolog subfamily B member 2 antibody
    • DnaJ homolog subfamily B member 2 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJB2 antibody
    • Heat shock 40 kDa protein 3 antibody
    • Heat shock protein J1 antibody
    • Heat shock protein neuronal DNAJ like 1 antibody
    • HSJ 1 antibody
    • HSJ1 antibody
    • HSPF3 antibody
    see all

Anti-DNAJB2 antibody 图像

  • Anti-DNAJB2 antibody (ab77322) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-DNAJB2 antibody (ab77322) at 5 µg/ml + A431 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab77322 was used as a capture antibody and detection limit for recombinant tagged DNAJB2 is approximately 0.3ng/ml.

Anti-DNAJB2 antibody (ab77322)参考文献

ab77322 has not yet been referenced specifically in any publications.

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