概述

  • 产品名称Anti-DNAJB11抗体
    参阅全部 DNAJB11 一抗
  • 描述
    兔多克隆抗体to DNAJB11
  • 特异性ab75107 detects endogenous levels of total DNAJB11 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from an internal region of human DNAJB11.

  • 阳性对照
    • IHC-P: human heart tissue. WB: extracts from HeLa cells, HUVEC cells, COLO cells and MCF-7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab75107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 1 J domain.
  • 翻译后修饰Contains high-mannose Endo H-sensitive carbohydrates.
    Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
    Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
  • 细胞定位Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABBP-2 antibody
    • ABBP2 antibody
    • APOBEC1 binding protein 2 antibody
    • APOBEC1-binding protein 2 antibody
    • DJ9 antibody
    • DJB11_HUMAN antibody
    • DnaJ (Hsp40) homolog subfamily B member 11 antibody
    • DnaJ homolog subfamily B member 11 antibody
    • DnaJ protein homolog 9 antibody
    • DNAJB11 antibody
    • EDJ antibody
    • ER associated DNAJ antibody
    • ER associated dnaJ protein 3 antibody
    • ER associated Hsp40 co chaperone antibody
    • ER-associated DNAJ antibody
    • ER-associated dnaJ protein 3 antibody
    • ER-associated Hsp40 co-chaperone antibody
    • ERdj3 antibody
    • ERJ3 antibody
    • ERj3p antibody
    • hDj-9 antibody
    • hDj9 antibody
    • HEDJ antibody
    • Human DnaJ protein 9 antibody
    • PWP1 interacting protein 4 antibody
    • PWP1-interacting protein 4 antibody
    see all

Anti-DNAJB11 antibody 图像

  • All lanes : Anti-DNAJB11 antibody (ab75107) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HUVEC cells
    Lane 3 : extracts from COLO cells
    Lane 4 : extracts from MCF-7 cells
    Lane 5 : extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Immunohistochemical analysis of DNAJB11 in paraffin embedded human heart tissue using 1/50 ab75107.
    Left image: untreated sample.
    Right image: sample treated with immunising peptide.

Anti-DNAJB11 antibody (ab75107)参考文献

This product has been referenced in:
  • Xu W  et al. Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis. Biol Reprod 89:142 (2013). Read more (PubMed: 24198121) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab75107.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"