概述

  • 产品名称Anti-DNAH12抗体
  • 描述
    兔多克隆抗体to DNAH12
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    C terminal amino acids 2993-3044 (IHGLYLDGAR WDRESGLLAE QYPKLLFDLM PIIWIKPTQK SRIIKSDAYV CP) of the Human DNAH12 protein (Q6ZR08).

  • 阳性对照
    • Human colon tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab122394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 功能Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
  • 序列相似性Belongs to the dynein heavy chain family.
  • 结构域Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • 细胞定位Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • 数据库链接
  • 别名
    • axonemal antibody
    • Axonemal beta dynein heavy chain 12 antibody
    • Axonemal dynein heavy chain 12-like protein antibody
    • Axonemal dynein heavy chain 7-like protein antibody
    • Ciliary dynein heavy chain 12 antibody
    • DHC3 antibody
    • DLP12 antibody
    • Dnah12 antibody
    • DNAH12L antibody
    • DNAH7L antibody
    • DNAHC3 antibody
    • DNHD2 antibody
    • DYH12_HUMAN antibody
    • Dynein heavy chain 12 antibody
    • Dynein heavy chain 7-like antibody
    • Dynein heavy chain domain-containing protein 2 antibody
    • HDHC3 antibody
    • HL19 antibody
    see all

Anti-DNAH12 antibody 图像

  • All lanes : Anti-DNAH12 antibody (ab122394) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • ab122394, at 1/50, staining DNAH12 in paraffin embedded Human colon tissue by immunohistochemistry.

Anti-DNAH12 antibody (ab122394)参考文献

ab122394 has not yet been referenced specifically in any publications.

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