Anti-DNA Polymerase theta抗体(ab77151)

概述

  • 产品名称Anti-DNA Polymerase theta抗体
    参阅全部 DNA Polymerase theta 一抗
  • 描述
    小鼠单克隆抗体to DNA Polymerase theta
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1609-1717 of Human DNA Polymerase theta

  • 阳性对照
    • Recombinant tagged human DNA Polymerase theta fragment (the immunogen).

性能

应用

Our Abpromise guarantee covers the use of ab77151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 198 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged DNA Polymerase theta is approximately 1 ng/ml when used as a capture antibody.

靶标

  • 功能Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.
  • 组织特异性Highly expressed in testis.
  • 序列相似性Belongs to the DNA polymerase type-A family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781A0112 antibody
    • DNA polymerase eta antibody
    • DNA polymerase theta antibody
    • DPOLQ_HUMAN antibody
    • POLH antibody
    • POLQ antibody
    • Polymerase (DNA directed) theta antibody
    • PRO0327 antibody
    see all

Anti-DNA Polymerase theta antibody 图像

  • Anti-DNA Polymerase theta antibody (ab77151) at 5 µg/ml + recombinant tagged human DNA Polymerase theta fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 198 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-DNA Polymerase theta antibody (ab77151)参考文献

ab77151 has not yet been referenced specifically in any publications.

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