重组Anti-DNA polymerase mu抗体[EPR10470(B)] (ab157465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DNA polymerase mu抗体[EPR10470(B)]
参阅全部 DNA polymerase mu 一抗 -
描述
兔单克隆抗体[EPR10470(B)] to DNA polymerase mu -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 293T, HeLa, Daudi and Jurkat cell lysates. IP: Jurkat cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10470(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 55 kDa. |
靶标
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功能
Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. -
组织特异性
Expressed in a number of tissues. Abundant in thymus. -
序列相似性
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 27434 Human
- Entrez Gene: 54125 Mouse
- Entrez Gene: 289757 Rat
- Omim: 606344 Human
- SwissProt: Q9NP87 Human
- SwissProt: Q9JIW4 Mouse
- Unigene: 596982 Human
- Unigene: 260194 Mouse
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别名
- DNA-directed DNA polymerase mu antibody
- DNA-directed DNA/RNA polymerase mu antibody
- DPOLM_HUMAN antibody
see all
图片
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All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLM knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDaLanes 1-4: Merged signal (red and green). Green - ab157465 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab157465 Anti-DNA polymerase mu antibody [EPR10470(B)] was shown to specifically react with POLM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265557 (knockout cell lysate ab258127) was used. Wild-type and POLM knockout samples were subjected to SDS-PAGE. ab157465 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution + immunoprecipitation pellet from Jurkat cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab157465 被引用在 2 文献中.
- Schimmel J et al. Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. EMBO J N/A:N/A (2017). WB ; Mouse . PubMed: 29079701
- Gassman NR et al. Hyperactivation of PARP triggers nonhomologous end-joining in repair-deficient mouse fibroblasts. PLoS One 7:e49301 (2012). PubMed: 23145148