This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-DNA polymerase mu抗体(ab3500)

概述

  • 产品名称Anti-DNA polymerase mu抗体
    参阅全部 DNA polymerase mu 一抗
  • 描述
    兔多克隆抗体to DNA polymerase mu

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 特异性This antibody has been tested by Western blot against a panel of human cell lines. It shows a band at close to the predicted band size of 55kD. However, we have no strong proof of specificity for DNA polymerase mu and so we have made this antibody available as a Fast-Track antibody.
  • 种属反应性

    预测可用于: Human
  • 免疫原

    Synthetic peptide:

    RRARVGSPSGDAAC

    , corresponding to amino acids 6-18 of Human POLM mu.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

  • 应用说明This product has been assayed by ELISA against 0.1 ug of the immunizing peptide. A 1/33000 to 1/135000 dilution of the antibody is recommended for this assay.

    This antibody has also been tested in Western blot using HeLa, A431, Jurkat and 293T lysates. A strong band of ~ 38kDa was detected, however no bands of the expected size (~55kDa) were seen. We would appreciate any customer feedback about this antibody.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
    • 组织特异性Expressed in a number of tissues. Abundant in thymus.
    • 序列相似性Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA-directed DNA polymerase mu antibody
      • DNA-directed DNA/RNA polymerase mu antibody
      • DPOLM_HUMAN antibody
      • FLJ35482 antibody
      • Pol iota antibody
      • Pol Mu antibody
      • Polm antibody
      • POLM protein antibody
      • Polymerase (DNA directed) mu antibody
      • Polymerase DNA directed mu antibody
      • Tdt N antibody
      • Tdt-N antibody
      • TdtN antibody
      • Terminal transferase antibody
      see all

    Anti-DNA polymerase mu antibody 图像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-DNA polymerase mu antibody (ab3500) at 1/500 dilution

      Lane 1 : HeLa nuclear lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : Jurkat whole cell lysate
      Lane 5 : HEK293 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Performed under reducing conditions.

      Exposure time : 2 minutes

    Anti-DNA polymerase mu antibody (ab3500)参考文献

    ab3500 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab3500.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"