Anti-DNA Polymerase lambda抗体(ab5954)


  • 产品名称Anti-DNA Polymerase lambda抗体
    参阅全部 DNA Polymerase lambda 一抗
  • 描述
    山羊多克隆抗体to DNA Polymerase lambda
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide: LGLPYREPAERDW, corresponding to C terminal amino acids 563-571 of Human DNA Polymerase lambda.

  • 常规说明GenBank Accession Number – NP_037406


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab5954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IHC-P: Use at a concentration of 5 µg/ml.
    Peptide ELISA: antibody detection limit dilution >1/32000.
    Western Blot: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1µg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
    • 组织特异性Expressed in a number of tissues. Abundant in testis.
    • 序列相似性Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BETA N antibody
      • BETAN antibody
      • DNA directed DNA polymerase lambda antibody
      • DNA polymerase beta 2 antibody
      • DNA polymerase beta-2 antibody
      • DNA polymerase kappa antibody
      • DNA polymerase kappa DNA polymerase beta N antibody
      • DNA polymerase lambda antibody
      • DNA polymerase lamda2 antibody
      • DPOLL_HUMAN antibody
      • EC,EC 4.2.99. antibody
      • FLJ46002 antibody
      • OTTHUMP00000020321 antibody
      • OTTHUMP00000020323 antibody
      • OTTHUMP00000059179 antibody
      • Pol beta2 antibody
      • POL KAPPA antibody
      • Pol Lambda antibody
      • POLKAPPA antibody
      • POLL antibody
      • Polymerase DNA directed lambda antibody
      see all

    Anti-DNA Polymerase lambda antibody 图像

    • ab5954 at 5ug/ml staining DNA Polymerase lambda in human small intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.

    Anti-DNA Polymerase lambda antibody (ab5954)参考文献

    This product has been referenced in:
    • Selak N  et al. The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta. Nucleic Acids Res 36:5166-79 (2008). WB ; Human . Read more (PubMed: 18682526) »

    See 1 Publication for this product

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    It shouldn't recognize polymerase beta. By BLAST analysis of the peptide we wouldn't predict any. But this has not been tested experimentally.