Anti-DNA Polymerase Kappa/POLK抗体(ab86076)


  • 产品名称Anti-DNA Polymerase Kappa/POLK抗体
    参阅全部 DNA Polymerase Kappa/POLK 一抗
  • 描述
    兔多克隆抗体to DNA Polymerase Kappa/POLK
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 675 and 725 of Human DNA Polymerase Kappa/POLK (NP_057302.1)

  • 阳性对照
    • HeLa or 293T whole cell lysate.



Our Abpromise guarantee covers the use of ab86076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
    • 组织特异性Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
    • 序列相似性Belongs to the DNA polymerase type-Y family.
      Contains 2 UBZ-type zinc fingers.
      Contains 1 umuC domain.
    • 结构域The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
    • 细胞定位Nucleus. Detected throughout the nucleus and at replication foci.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DinB homolog 1 (E. coli) antibody
      • DINB protein antibody
      • DINB1 antibody
      • DINP antibody
      • DNA damage inducible protein b antibody
      • DNA polymerase kappa antibody
      • Polk antibody
      • POLK_HUMAN antibody
      • POLQ antibody
      • Polymerase (DNA directed) kappa antibody
      • polymerase, DNA, kappa antibody
      see all

    Anti-DNA Polymerase Kappa/POLK antibody 图像

    • All lanes : Anti-DNA Polymerase Kappa/POLK antibody (ab86076) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size : 99 kDa

    Anti-DNA Polymerase Kappa/POLK antibody (ab86076)参考文献

    ab86076 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86076.
    Please use the links above to contact us or submit feedback about this product.