概述

  • 产品名称Anti-DNA Polymerase Kappa/POLK抗体
    参阅全部 DNA Polymerase Kappa/POLK 一抗
  • 描述
    小鼠单克隆抗体to DNA Polymerase Kappa/POLK
  • 经测试应用适用于: WB, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (proprietary-tag) corresponding to Human DNA Polymerase Kappa/POLK aa 1-472.
    Sequence:

    MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFY GNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLS NTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRA AMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEA YLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISP LLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFR IEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISL GLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKER LKVLYFDMVSLVFKFFNSKMLP


    Database link: AAH50718

性能

应用

Our Abpromise guarantee covers the use of ab57070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 99 kDa.
IHC-P Use a concentration of 3 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

Detection limit for recombinant tagged DNA Polymerase Kappa/POLK is approximately 0.3 ng/ml as a capture antibody

ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
  • 组织特异性Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
  • 序列相似性Belongs to the DNA polymerase type-Y family.
    Contains 2 UBZ-type zinc fingers.
    Contains 1 umuC domain.
  • 结构域The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • 细胞定位Nucleus. Detected throughout the nucleus and at replication foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DinB homolog 1 (E. coli) antibody
    • DINB protein antibody
    • DINB1 antibody
    • DINP antibody
    • DNA damage inducible protein b antibody
    • DNA polymerase kappa antibody
    • Polk antibody
    • POLK_HUMAN antibody
    • POLQ antibody
    • Polymerase (DNA directed) kappa antibody
    • polymerase, DNA, kappa antibody
    see all

Anti-DNA Polymerase Kappa/POLK antibody 图像

  • Anti-DNA Polymerase Kappa/POLK antibody (ab57070) at 1 µg + HeLa nuclear lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 99 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue, staining DNA Polymerase Kappa/POLK with ab57070 at 3 µg/ml.

  • Immunofluorescence of HeLa cells, staining DNA Polymerase Kappa/POLK with ab57070 at 10 µg/ml.

  • All lanes : Anti-DNA Polymerase Kappa/POLK antibody (ab57070) at 5 µg/ml

    Lane 1 : DNA Polymerase Kappa/POLK-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-Mouse IgG
    Developed using the ECL technique

    Predicted band size : 99 kDa
    Additional bands at : 54.1 kDa (possible isoform).
  • Detection limit for recombinant tagged DNA Polymerase Kappa/POLK is approximately 0.3 ng/mL as a capture antibody

Anti-DNA Polymerase Kappa/POLK antibody (ab57070)参考文献

This product has been referenced in:
  • Ziv O  et al. Identification of novel DNA-damage tolerance genes reveals regulation of translesion DNA synthesis by nucleophosmin. Nat Commun 5:5437 (2014). WB . Read more (PubMed: 25421715) »

See 1 Publication for this product

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